QUALITATIVE AND QUANTITATIVE ANALYSIS OF COLLAGEN PROTEIN
Authors
Shakhnoza Matsafayeva, Anvar Normatov, Nazarov Golib, Niyozov Khasan, Asadjon Otajonov
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This research mainly presents the results of the analysis of the quality and quantity of proteins extracted from cattle hide. The study focuses on the processing of cattle hide, which is considered an industrial waste, and the proper organization of collagen extraction. Additionally, the composition and purity of the extracted protein are assessed. The total nitrogen content in the sample was measured using the Kjeldahl method, and the total percentage of protein was calculated using a conversion factor (usually 6.25, or a factor adjusted to the research conditions). Furthermore, the amount of water-soluble proteins in the mixture was analyzed using the Lowry method at a wavelength of 570 nm with a UV-Vis 1900i instrument. The individual amino acids in the mixture were quantitatively and qualitatively analyzed using HPLC at a wavelength of 269.4 nm.
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Authors
Shakhnoza Matsafayeva, Anvar Normatov, Nazarov Golib, Niyozov Khasan, Asadjon Otajonov
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References:
Mokrejs, P.; Langmaier, F.; Mladek, M.; Janacova, D.; Kolomaznik, K.; Vasek, V. (2009). Extraction of collagen and gelatine from meat industry by-products for food and non-food uses. Waste Management & Research, 27(1), 31–37. doi:10.1177/0734242X07081483.
Avery, N.C. & Bailey, A.J. (1995). An efficient method for the isolation of intramuscular collagen. Meat Science, 41, 97–100. Briassoulis, D. (2004). An overview on mechanical behaviour of biodegradable agricultural films. Journal of Polymers and the Environment, 12, 65–81.
British Standard 757:1975 (Methods for Sampling and Testing Gelatine) British Standards Institution, London.
Buljan, J., Reich, G. & Ludvik J. (2000). Mass balance in leather processing. In: Proc. of the Centenary Congress of the IULTC, London, pp. 138–140. UNIDO, August 9th 2000. US/RAS/92/120-Regional Programme for Pollution Control in the Tanning Industry in Southeast Asia.
Dubois, M. (1992). Collagen. In: Martini, M.C. & Seiller, M. (eds.): Active Additives in Cosmetology, pp. 73–99. Lavoisier Tecdoc, Paris.
Ericson, C.M. (1993). Lipid extraction from channel muscle by composition solvent system. Journal of Food Science, 58, 84–87.
Gault, N.F.S. & Lawrie, R.A. (1980). Efficiency of protein extraction and recovery from meat industry by-products. Meat Science, 4, 167–190.
Eduard Roether, Darmstadt, Germany. Hwang, K.T. & Regenstein, J.M. (1993). Characteristics of mackerel mince lipids hydrolysis. Journal of Food Science, 58, 79–82.
Langmaier, F., Mladek, M., Kolomaznik, K. & Maly, A. (2002a). Degradation of chromed leather waste hydrolysates for the production of surfactants. Tenside Surfactants Detergents, 39, 31–34.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. (2011). Protein measurement with Folin phenol reagent. J. Biol. Chem.
Methods of control. Chemical factors. Guide for methods of quality control and safety of biologically active food additives. Guide R 4.1.1672-03. Moscow: Federal Center for State Sanitary and Epidemiological Surveillance, Ministry of Health of Russia, 2004.
Steven A., Cohen Daviel J. (1988). Amino acid analysis utilizing phenylisothiocyanate derivatives. Journal of Analytical Biochemistry, 17(1), 1–16.
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