IDENTIFICATION OF NEW BACILLUS VELEZENSIS ENDOPHYTIC STRAINS – PROBIOTIC AGENTS FOR SOYBEAN SYMBIOTIC SYSTEMS DEVELOPMENT
Authors
Shevchuk Nadiia, Tytova Liudmyla

Share
Annotation
The aim of the study was to identify soybean endophytic bacteria and determine the prospects for their use in soybean cultivation. It was found that the isolated strains belong to Gram-positive spore-forming bacteria that ferment a wide range of carbohydrates. Based on whole-genome sequencing and phylogenetic analysis, the endophytes were identified as Bacillus velezensis IMV B-8134 and IMV B-8135. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JBPUKF000000000 and JBRIKE000000000, BioProject PRJNA1294768. The application of the isolated endophytic strains together with nodule bacteria in soybean cultivation under biotic and abiotic stresses showed a positive effect on adaptation and productivity of plants. This provides grounds for further research on strains as promising for the creation of biological agents in soybean cultivation.
Keywords
Authors
Shevchuk Nadiia, Tytova Liudmyla

Share
References:
Afzal, I., Shinwari, Z. K., Sikandar, S., Shahzad, S. (2019). Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research, 221, 36–49.
Burragoni, S. G. & Jeon, J. (2021). Applications of endophytic microbes in agriculture, biotechnology, medicine, and beyond. Microbiological Research, 245, 126691.
Cheng, Y., Jiang, X., He, X., Wu, Z., Lv, Q., Zhao, S., ... & Liu, J. (2025). Bacillus velezensis 20507 promotes symbiosis between Bradyrhizobium japonicum USDA110 and soybean by secreting flavonoids. Frontiers in Microbiology, 16, 1572568.
Gordon, R.E., Haynes, W.C., Pang, C. H.-N. (1973). The Genus Bacillus. Agriculture Handbook No. 427. Agricultural Research Service United States Department of Agriculture, pp. 9-11.
Khatarkar, N., Kalpana, T., Geeta, P. (2017). Isolation, Screening And Characterization Of Amylase Producing Bacteria From Soil. Asian Journal of Pharmaceutical Education and Research. 6 (2):30-44.
Kondo, T., Sibponkrung, S., Hironao, K. Y., Tittabutr, P., Boonkerd, N., Ishikawa, S., ... & Yoshida, K. I. (2023). Bacillus velezensis S141, a soybean growth-promoting bacterium, hydrolyzes isoflavone glycosides into aglycones. The Journal of General and Applied Microbiology, 69 (3), 175-183.
Ruiz-Garcia, C., Bejar, V., Martinez-Checa, F., Llamas, I., Quesada, E. (2005). Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain. International Journal of Systematic and Evolutionary Microbiology, 55(1), 191-195.
Santoyo, G., Moreno-Hagelsieb, G., del Carmen Orozco-Mosqueda, M., Glick, B.R. (2016). Plant growth-promoting bacterial endophytes. Microbiol. Res. 183, 92–99.
Sibponkrung, S., Kondo, T., Tanaka, K., Tittabutr, P., Boonkerd, N., Yoshida, K. I., Teaumroong, N. (2020). Co-inoculation of Bacillus velezensis strain S141 and Bradyrhizobium strains promotes nodule growth and nitrogen fixation. Microorganisms, 8 (5), 678.
Shevchuk, N.V., Tytova, L.V., Iutynska, G.O., Sergiienko, V.G., Katriі, V.B. (2025). Influence of rhizobia and endophytic bacteria on adaptation and productivity of Glycine max (L.) Merr. under hyperthermіa and drought conditions. Microbiological journal, 87 (5), 12-25.
Shevchuk, N.V., Tytova, L.V., Sergiienko, V.G., Iutynska, G.O. (2025). Phytoprotective potential of soybean endophytic microsymbionts. Microbiological journal, 87 (6), pp. 27-40.
Yoon, S. H., Ha, S. M., Lim, J., Kwon, S., & Chun, J. (2017). A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek, 110 (10), 1281-1286.
