GC-MS analysis of bioactive compounds from Pleurotus ostreatus
Authors
Nilufar Tursunova, Kamolov Luqmon, Nurbek Umirov
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In this study, the ethyl acetate extract of Pleurotus ostreatus mushroom was analyzed using gas chromatography–mass spectrometry (GC–MS, EI ionization). The main objective of the study was to identify lipophilic secondary metabolites in the mushroom and evaluate their structural properties. According to the results of GC–MS analysis, three main bioactive components were identified in the extract - ethyl linoleate, ergosta-3,5,7,22-tetraene (22E) and ergosta-5,7,22-trien-3-ol acetate, and their molecular ions and fragmentation patterns were confirmed to match the database. Ethyl linoleate was characterized by the McLafferty rearrangement characteristic of fatty acid ethyl esters, while sterol derivatives were identified by fragment ions characteristic of the ergosterol skeleton. The results obtained show that the ethyl acetate fraction of P. ostreatus is rich in bioactive sterols and fatty acid esters, allowing this mushroom to be evaluated as a promising raw material for the development of pharmaceutical, nutraceutical, and functional food products.
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Authors
Nilufar Tursunova, Kamolov Luqmon, Nurbek Umirov
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References:
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