Academic publishing in Europe and N. America

Archive Publication ethics Submission Payment Contacts
In the original languageTranslation into English

ASSESSMENT OF RELATIONSHIP IN CHICKPEA (CICER ARIENTINUM L.) BASED ON PRODUCTIVITY CHARACTERISTICS AND SSR MARKERS IN AZERBAIJAN

Authors

Saida Hasanova, Sudabe Hasanova, Ramis Aliyev

Rubric:Biology
603
2
Download articleQuote
603
2

Annotation

Genetic diversity and marker–trait associations related to yield components were assessed in 43 chickpea (Cicer arietinum L.) accessions from a germplasm collection using winter-sown seeds in Azerbaijan. The accessions originated from Azerbaijan, Iran, and the International Center for Agricultural Research in the Dry Areas (ICARDA). Molecular genotyping was conducted using ten simple sequence repeat (SSR) markers. Yield and its components were evaluated across eight traits in field experiments. Correlation analysis revealed that yield was primarily influenced by plant height, the number of branches, and the formation of a high number of pods. These traits showed their highest values in chickpea genotypes. SSR analysis detected between four and seven alleles per marker. The highest levels of polymorphism were observed in markers TA46 (PIC = 0.88), H1A12 (PIC = 0.84), and H3E04 (PIC = 0.82), while the lowest were recorded in NCPGR6 (PIC = 0.42) and H4E04 (PIC = 0.44). Cluster analysis grouped the accessions into five distinct clusters. Several unique alleles identified through this analysis may serve as molecular identifiers (passport markers) for specific genotypes. These informative markers can be useful for chickpea breeding programs focused on yield improvement. Breeding lines with sufficient genetic distance from this study can be used as appropriate parents to get more heterotic recombinants. Correlation analysis was performed between 10 SSR primers and yield components,  informative SSR primers related to important trait of plant height were found.

Keywords

SSR
molecular markers
cluster
correlation.

Authors

Saida Hasanova, Sudabe Hasanova, Ramis Aliyev

References:

BHATTARAI, G., SHI, A., KANDEL, D.R., SOLIS-GRACIA, N., DA SILVA, J.A., et al. (2021): Genome-wide simple sequence repeats (SSR) markers discovered from whole-genome sequence comparisons of multiple spinach  accessions. Sci. Rep., 11:9999. [CrossRef]

FAYAZ, H., MIR, A.H., TYAGI, S., WANI, A.A., JAN, N., et al. (2021): Assessment of molecular genetic diversity of 384 chickpea genotypes and development of core set of 192 genotypes for chickpea improvement programs. Genet. Resour. Crop Evol, 69:1193-1205. [CrossRef]

HASANOVA, S.Q., MAMMADOVA, A.D., ALIYEV, R. (2023): Study of chickpea genotypes (Cicer arietinum l.) resistance to fusarium disease with DNA markers. European journal of natural history, 6: 4-9. ISSN 2073-4972.

JHA, U.C., JHA, R., THAKRO, V., KUMAR, A., GUPTA, S., et al. (2021): Disceming molecular diversity and association mapping for phenological, physiological and yield traits under high temperature stress in chickpea (Cicer arietinum L.). J. Genet., 100:4-10. [CrossRef] 

LASSNER, M.W., PETERSON, P. (1989): Yoder JI Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Mol. Biol. Rep., 7:116-128. [CrossRef] 

LIU, K., MUSE, S.V. (2005): Power Marker: An integrated analysis environment for genetic marker analysis. Bioinformatics, 21: 2128-2129. [CrossRef] [PubMed]

MAZKIRAT, S., BAITARAKOVA, K., KUDAYBERGENOV, M., BABISSEKOVA, D., BASTAUBAYEVA, S., et al. (2023): SSR Genotyping and Marker-Trait Association with Yield Components in a Kazakh Germplasm Collection of Chikpea (Cicer arietinum L.). Biomolecules, 13:1722. https://doi.org/10.3390/ biom1312722 R_Core_Team R (2023). A Language and Environment for Statistical Computing; R Foundation for Statistical Computing: Vienna, Austria; Aviable online: https://www.R-project.org (accesed on 30 October 2023)

SEREKPAYEV, N., POPOV, V., STYBAYEV, G., NOGAYEV, A., ANSABAYEVA, A. (2016): Agroecological aspects of chickpea groving in the dry steppe zone of Akmola region, Northern Kazakhstan. Biotechol. Res. Asia 13:1341. [CrossRef]

SARI, D., SARI, H., IKTEN, C., TOKER, C. (2023): Genome-wide discovery of di-nucleotide SSR markers based on whole genome re-sequencing data of Cicer arietinum L. and Cicer reticulatum Ladiz. Sci. Rep. 13:10351. [CrossRef]

SERROTE, C.M.L., REINIGER, L.R.S., SILVA, K.B., RABAIOLLI, S.M.D., STEFANEL, C.M. (2020): Determining the polymorphism information content of a molecular marker. Gene, 726:144175. [CrossRef] [PubMed]

SING, R.K., SING, C., AMBIKA, C.B.S., MAHTO, R.K., PATIAL, R., et al. (2022): Exploring chikpea germplasm diversity for broadening the genetic base utilizing genomic resources. Front. Genet. 13:905771. [CrossRef]

VARSHNEY, R., THUDI, M., UPADHYAYA, H., DWIVEDI, S., UDUPA, S., et al. (2014): ASSR kit to study genetic diversity in chickpea (Cicer arietinum L.). Plant Genet. Resour. 12: 118-120. [CrossRef]

VARSHNEY, R.K., GRANER, A., SORRELLS, M.E. (2005): Genic microsatellite markers in plants: Features and applications. Trends Biotechnol. 23:48-55. [CrossRef]

ZHOU, R., YANG, B., QI, Z., XIN, D., et al. (2021): Analysis of the genetic diversity of grain legume germplasm resources in China and the development of universal SSR primers. Biotechnol. Equip. 35:1706-1721. [CrossRef]

Other articles of the issue

Nataliia Kravchenko Building a recruitment system from scratch in a startup: rapid scaling strategies
Download article in PDF173 views
cc-license
About us Journals Books
Publication ethics Terms of use of services Privacy policy
Copyright 2013-2025 Premier Publishing s.r.o.
Praha 8 - Karlín, Lyčkovo nám. 508/7, PSČ 18600, Czech Republic pub@ppublishing.org