Fine-Tuning Chromoprotein Expression for Bioproduction in Halomonas spp.
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Ruiyan Huang
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As bioproduction, especially microbial production, of substances became spotlighted, attention has been raised on developing tools to combat the unpredictable cellular synthetic activities that are often posed by the microbial cell factories. Fine-tuning tools, as one way to ameliorate cell factories for production, have become popular and effective for improving biosynthesis processes by achieving relatively stable regulatory purposes. In this study, a tunable expression system was created and utilized to control the level of expression of five single genes in the non-model bacteria Halomonas bluephagenesis, a member of Halomonas spp. and a sustainable and low-cost bioengineering and biomanufacturing platform for “next-generation industrial biotechnology” (NGIB). To better visualize results of the inducible flux regulation of this multiple inducible expression system which is designed to control five genes simultaneously, chromoprotein single genes were expressed and regulated through various combinations of induction concentration. The results demonstrated the system’s ability to regulate expression of multiple individual genes at the same time. The intensely visible chromoprotein production of varied colors and the colorful bacteria paintings from the visual results also proved the viability of this system in Halomonas bluephagenesis, the utilization of which contributes to a more cost-effective and waste-reducing solution to resource waste and cost issues of current industrial biotechnology. Overall, this study reveals the feasibility of the desirable tuning system for efficacious expression regulation of target genes and allows for further exploration and metabolic flux optimization in the bioproduction of other substances to allow more stable bioproduction processes in synthetic biology.
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Ruiyan Huang
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