The stability of glucose and triglyceride analysis in gel tubes at 2-8⁰C

Introduction:  Studies have shown that there are important, significant relationships between the values ​​of different analytes with different physical variables such as temperature, measurement time, and centrifugation time. In this paper, the relationship that may exist between the concentration values ​​of two analytes such as blood glucose and triglycerides with the storage temperature of the samples has been studied.

This study aimed to determine the analyte stability of venous blood samples in serum gel tubes stored at 2-8⁰C.

Methods: 30 healthy adult volunteers take part in the study. Blood was collected in tubes with gel (Clot activator). All samples were allowed to clot at room temperature for 30 min. They were centrifuged CAPP  CENTRIFUGE at 3500 RPM and 2 analytes were analyzed: Triglycerides and Fasting Blood Glucose. These values ​​were determined as control values. Serums were stored at 2-8⁰ C. Measurements were repeated after 1 week.

Results: 18 men and 12 women participated in the study. Triglycerides control values ​​range from 54-149mg/dl average (86.58mg/dL).

Fasting blood glucose values ​​were in the range of 77-91.8mg/dl, with an average value (of 80.23 mg/dL).

After keeping the serum at a temperature of 2-8⁰C for 1 week, the following results were obtained:

• Triglyceride values ​​ranged from 60.3-117 mg/dl, mean of value (92.25mg/dl)
• Glucose values ​​ranged from 53-124.4mg/dl on average (83.7mg/dL)

Conclusions: There is a statistically significant relationship between the storage temperature of the sample and the concentration of the Triglyceride analyte (p- 0.00), while for the comparison of Glucose control and Glucose values ​​after one week, they are not statistically significant (p= 0.2).