Preparation and Comprehensive Characterization of an Aldehyde-Modified Hyaluronate-Containing Polysaccharide Fraction for Hydrogel Systems
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Hyaluronic acid (HA) is widely used in injectable biomaterials because of its favorable physicochemical properties. In this study, a hyaluronate-containing polysaccharide fraction (HCPF) was isolated from chicken skin and modified by sodium periodate oxidation. The purified HCPF yield was 0.31% (w/w). FT-IR confirmed characteristic hyaluronate-type polysaccharide bands, while a 2% (w/v) solution showed pseudoplastic behavior with a zero-shear viscosity of 0.449 ± 0.003 Pa·s and a viscoelastic crossover at 14.6 rad/s. Periodate oxidation resulted in a degree of oxidation of 50.2 ± 1.8% and an aldehyde content of 2.65 ± 0.08 mmol/g. GPC analysis showed a decrease in molecular weight from 544 ± 28 to 149 ± 12 kDa. These results demonstrate successful preparation of an aldehyde-modified HCPF suitable as a precursor for Schiff-base or hydrazone hydrogel systems.
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