COMPREHENSIVE ASSESSMENT OF THE IMPACT OF VARIOUS FACTORS ON THE QUALITY AND YIELD OF COLLAGEN
Authors
Radjabov Otabek Iskandarovich, Yariev Olimjon Oltinovich, Azimova Luiza Bakhtiyarovna, Filatova Albina Vasilievna, Djurabaev Djalol Turgunbaevich
Share
Annotation
In this article, the optimal conditions for the extraction of collagen with its natural triple helical structure were studied by chemical hydrolysis of renewable raw materials - cattle hides. Changes in the amount of chemicals in the hide at different stages of hydrolysis were determined, and the optimal concentration of the alkaline-salt hydrolysis solution and the hydrolysis temperature were determined. According to the results of the study, when using a solution of 10% NaOH and 100 g/l Na2SO4 at a temperature of 20°C, natural collagen with a high yield of up to 83% was obtained. When the effect of the citrate buffer pH environment was studied, the pH=4 condition was found to be the most optimal. By measuring the UV spectrum of the isolated collagen, it was determined that it had a natural structure, and by finding the total nitrogen content, it was determined that it had a high purity level (99.6%).
Keywords
Authors
Radjabov Otabek Iskandarovich, Yariev Olimjon Oltinovich, Azimova Luiza Bakhtiyarovna, Filatova Albina Vasilievna, Djurabaev Djalol Turgunbaevich
Share
References:
Liu D., Nikoo M., Boran G., Zhou P., Regenstein J.M. Collagen and gelatin // Annual Review of Food Science and Technology. 2015. 6. P. 527-557.
Shoulders M.D., Raines R.T. Collagen structure and stability // Annual Review of Biochemistry. 2009. 78. P. 929-958.
Friess W. Collagen-biomaterial for drug delivery // European Journal of Pharmaceutics and Biopharmaceutics. 1998. 45(2). P. 113-136.
Sionkowska A. Current research on the blends of natural and synthetic polymers as new biomaterials // Progress in Polymer Science. 2011. 36(9). P. 1254-1276.
Zhang Z., Li G., Shi B. Physicochemical properties of collagen, gelatin, and collagen hydrolysates derived from bovine limed split wastes. Journal of the Society of Leather Technologists and Chemists, 2006, 90: P. 23-28.
Woo H, Jeong G.A., Choi H., Lee C.J. Characterization of Low-Molecular-Weight Collagen from Korean Native Chicken Feet Hydrolyzed Using Alcalase // J Microbiol Biotechnol. 2023. May 28;33(5), P. 656-661.
Mo C., Wang Q., Li G., Dong W., Liang F., Wu C., Wang Z., Wang Y. Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca // Mar. Drugs. 2023. 21. 159.
Other articles of the issue
