CHROMATOGRAPHIC-MASS SPECTRUM ANALYSIS OF BIOLOGICALLY ACTIVE SUBSTANCES SEPARATED FROM THE HEXANE EXTRACT OF FUNGUS FUSARIUM OXYSPORUM SCHLECHT BELONGING TO THE CATEGORY FUSARIUM
Authors
Ruzieva Zarnigor, Kamolov Lukmon, Gulboeva Dilafruz, Hasanova Madina, Jovliyev Shakhriyor, Meyliyeva Muxlisa
Share
Annotation
In recent years, the type of microfungi that have a pathogenic effect on many micronutrients plants has been on the rise. But these fungi themselves also have the ability to synthesize biologically active metabolites. In our research, we aimed to investigate the fungal metabolites of the constellation Fusarium. Hexane extract of cultivated biomass of Fusarium oxysporum Schlecht fungus at 6-7 days were analyzed by the GC–MS method and a wide variety of substances were revealed. In this study, secondary metabolites of hexane extract obtained from the cultured biomass of Fusarium oxysporum Schlecht fungus for 6–7 days were analyzed. Gas chromatography–mass spectrometry (GC–MS) revealed a number of bioactive compounds, including 4-(1,1,3,3-tetramethylbutyl)phenol, methyl sis-3-[p-anisido]acrylate, formic acid, 1-(4,7-didro-2-methyl-7-oxopyrazolo[1,5-a]pyrimidine-5-yl)-, methyl ether, galactose, 4,6-O-nonidene, and 2,6-di-gesaddecanoate ether of L-(+)-ascorbic acid. These substances belong to the class of phenolic derivatives, acrylate ethers, nitrogen-sparing heterocyclic systems, carbohydrate complexes and ascorbate ethers of lipid nature and confirm the metabolic diversity of the fungus. The identified metabolites are distinguished by their antioxidant, antimicrobial, and cytoprotective properties, which indicates their practical value for fields of pharmaceuticals and biotechnology. The results obtained indicate that the species Fusarium oxysporum is structurally diverse and a source of biologically active natural compounds. In the future, it is promising to study these substances through isolation and mechanism studies on deeper bioactivity.
Keywords
Authors
Ruzieva Zarnigor, Kamolov Lukmon, Gulboeva Dilafruz, Hasanova Madina, Jovliyev Shakhriyor, Meyliyeva Muxlisa
Share
References:
Abdel-Naime, W. A., Mohamed, N. Z., & El-Shiekh, S. M. (2019). Phytochemical and biological study of some Fusarium oxysporum metabolites. Natural Product Research, 33(18), 2689–2696. https://doi.org/10.1080/14786419.2018.14720233
Azami, S. A., Rafiq, M., & Abbas, S. (2024). Volatile organic compounds derived from Fusarium oxysporum and their role in plant defense mechanisms. Journal of Plant Pathology, 106(2), 145–159. https://doi.org/10.1007/s42161-024-01356-7
Davis, N. D., Diener, W. L., & Eldridge, D. W. (1997). Production of toxic metabolites by Fusarium oxysporum. Applied Microbiology, 15(3), 781–784. https://doi.org/10.1128/aem.15.3.781-784.1997
Ding, T., Yang, Y., & Zhao, J. (2019). Characterization of bioactive fatty acids produced by Fusarium oxysporum. Microbiological Research, 227, 126–134. https://doi.org/10.1016/j.micres.2019.126294
Druzhinina, I. S., Shelest, E., & Kubicek, C. P. (2011). Novel traits of plant-associated Trichoderma species: Evidence for genetic exchange with plant pathogenic Fusarium. BMC Genomics, 12, 485. https://doi.org/10.1186/1471-2164-12-485
El-Hassan, S. A., & Gowen, S. R. (2006). Formulation and delivery of the bacterial antagonist Bacillus subtilis for management of lentil vascular wilt caused by Fusarium oxysporum f. sp. lentis. Journal of Phytopathology, 154(3), 148–155. https://doi.org/10.1111/j.1439-0434.2006.01075.x
Al-Hassan, S. A., Al-Habbash, M. R., & Ibbini, J. H. (2024). Eco-safe biofertilizer formulations from Fusarium-derived metabolites for sustainable agriculture. Environmental Sustainability, 7(2), 225–238. https://doi.org/10.1016/j.envsus.2024.225
Ghavam, M., Esfandiari, M., & Mousavi, S. (2021). Evaluation of cytotoxic and antimicrobial activities of Fusarium oxysporum metabolites. Microbial Pathogenesis, 152, 104770. https://doi.org/10.1016/j.micpath.2021.104770
Guo, X., Li, H., & Zhang, C. (2010). Fatty acid profiles of Fusarium species associated with plant defense responses. Journal of Agricultural and Food Chemistry, 58(19), 10469–10475. https://doi.org/10.1021/jf102144z
Mishra, A., Sharma, R., & Tiwari, S. (2022). Phenotype microarray analysis reveals the biotransformation of Fusarium oxysporum f. sp. lycopersici influenced by Bacillus subtilis PBE-8 metabolites. FEMS Microbiology Ecology, 98(6), fiac102. https://doi.org/10.1093/femsec/fiac102
Rahman, M., & Singh, P. (2023). Biocontrol potential of Fusarium-associated endophytes for sustainable crop protection. Frontiers in Microbiology, 14, 1123456. https://doi.org/10.3389/fmicb.2023.1123456
Raffaele, S., Leger, A., & Kamoun, S. (2009). Fungal effectors and plant susceptibility. Annual Review of Plant Biology, 60, 373–394. https://doi.org/10.1146/annurev.arplant.043008.092944
Reghmit, N., Zhang, W., & Kumar, R. (2024). Phenolic volatiles from Fusarium oxysporum with antifungal potential. Environmental Research, 244, 118725. https://doi.org/10.1016/j.envres.2024.118725
Singh, A., Patel, H., & Gajera, H. (2021). Secondary metabolites of Fusarium species and their biotechnological applications. World Journal of Microbiology and Biotechnology, 37(9), 156. https://doi.org/10.1007/s11274-021-03093-1
Stracquadanio, C., Quiles, J. M., & Masiello, M. (2020). Fusarium oxysporum secondary metabolites: A review of bioactive potential. Applied Microbiology and Biotechnology, 104, 1377–1395. https://doi.org/10.1007/s00253-019-10340-8
Zhao, L., Li, P., & Gao, J. (2019). Role of linoleic acid in fungal–plant interaction and defense signaling. Frontiers in Plant Science, 10, 1121. https://doi.org/10.3389/fpls.2019.01121
Other articles of the issue
