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European Science Review, Issue 3-4/2018

METHODOLOGICAL APPROACHES TO MONITORING MICROBIAL COMMUNITIES OF AGRICULTURAL LANDSCAPE OF THE STEPPE ZONE

DOI: https://doi.org/10.29013/ESR-18-3.4-235-238

Pages: 235 - 238

Authors: Zhurlov O. S., Grudinin D. A., Mushinskiy A. A., Yakovlev I. G.

Abstract: Abstract: The article analyzes the methodological features of sampling soil for agrochemical, microbiological and metagenomic analysis. The possibility of integrating soil sampling methods on the basis method for sampling soil from equidistant points with GPS positioning is discussed, as a single methodical technique, most accurately reflecting the features of the distribution of physical and chemical properties and microbial communities of soil. Standardization of soil sampling methods for physicochemical, microbiological and metagenomic research is necessary for the construction of a GIS “Microbial communities of agricultural landscape”.

Keywords: Keywords: agroecology, bacterial communities, agricultural landscape, geographic information system (GIS).

Bibliography:
1. Arinushkina E. V. Guidelines for the chemical analysis of soils. – Moscow: Moscow State University, – 1970. – 487 p.
2. GOST 17.4.3.01–83. (ST SEV 3847–82). Protection of Nature. Soil. General requirements for sampling. Enter.
from 01.07.84 to 01.01.89. – Moscow: Publishing Standards, – 1983. – 2 p.
3. GOST 17.4.4.02–84. Protection of Nature. Soil. Methods of selection and preparation of samples for chemical,
bacteriological, helminthological analysis. Enter. from 01/01/86. – Moscow: Publishing Standards, – 1984. – 15 p.
4. Methods of soil microbiology and biochemistry. Edited by D. G. Zvyagintseva. – M. – 1991. – 303 p.
5. Dobrovolskaya T. G., Golovchenko A. V., Pankratov T. A., Lysak L. V., Zvyagintsev D. G. Assessment of bacterial
diversity of soils: the evolution of approaches and methods. Soil Science. – 2009. – No. 10. – P. 1222–1232.
6. Methodological recommendations sampling of soils, soils, bottom sediments, silt, sewage sludge, industrial sewage
sludge, production and consumption wastes. PND F 12.1: 2: 2: 2: 2.3: 3.2–03. – Moscow, – 2014.
7. Andronov E. E., Pinaev A. G., Pershina E. V., Chizhevskaya E. P. DNA isolation from soil samples (methodical
instructions). SPb: ARRIAM RAAS. – 2011. – 27 p.
8. Fierer N., Morse J. L., Berthrong S. T. et al. Environmental controls on the landscape–scale biogeography of stream
bacterial communities. Ecology. – 2007. – Vol. 88. – P. 2162–2173.
9. Ebrahimi A., Or D. Microbial community dynamics in soil aggregates shape biogeochemical gas fluxes from soil
profiles – upscaling an aggregate biophysical model. Glob Chang Biol. – 2016. – Vol. 22. – P. 3141–3156. URL:
http://dx.doi.org/10.1111 / gcb.13345
10. Lauber C. L., Strickland M. S., Bradford M. A., Fierer N. The influence of soil properties on the structure of the
bacterial and fungal communities across land–use types. Soil Biol. Biochem. – 2008. – Vol. 40. – P. 2407–2415.
URL: http://dx.doi.org/10.1016/j.soilbio.2008.05.021