Pages: 126 - 129
Authors: Khadjibaev А. M., Tilyakov A. B., Magrupov B. A., Urazmetova M. D., Ubaydullaev B. S.
Abstract: Aim of investigation. Ground of embryonal allofibroblasts in the process of cartilaginous tissue regeneration. Material and methods. Investigation is based on the study the results of stimulation cartilaginous tissue regeneration process in the conditions of embryonal allofibroblasts application in 24 experimental sexually mature rabbits in which the model of symphysis pubis rupture with its following recovery have been used. Pieces of cartilaginous tissue have been fixed in 10% neutral formalin and embedded in paraffin. Histological sections were colored with hematoxylin, eosin and by Van-Gieson. Experiments duration — 7, 14, 21and 30 days. Results. After pelvic bones symphysis pubis rupture, cartilaginous tissue is undergone alternative changes with following formation of lobulose chondromatous nodules of different sizes and sclerosis of surrounding muscular and osseous tissue. In the second series of experiment after pelvis symphysis pubis rupture and symphysis recovery with the help of cerclage, morphologic investigation results testify formation of mixed initial callus consisting of connective and chondromatous tissue elements. In the third series of experiment embryonal allofibroblasts were introduced into symphysis pubis rupture defect after its recovery by cerclage. It has been shown that within 4 weeks of experiment connective tissue having intensive basophil stain is formed in the area of symphysis pubis rupture. Connective tissue formation wit following formation of cartilaginous cells have been also noted in surrounding sift tissue structures. Conclusion. Cartilaginous tissue formation process in the area of symphysis pubis rupture in experiment testifies stimulating influence of allogenic fibroblasts on cartilaginous tissue regeneration process in the experiment and it creates premises for applying this method in clinical practice.
Keywords: embryonal allofibroblasts; cartilaginous tissue regeneration.
1. Arseniev G., Berchenko D. S., Urazguldeev G. N. Actual issues of clinical and experimental medicine. – 2007. – 379–381.
2. Bobro L. I. Fibroblasts and their role in tissue reactions. Arch pathology. – 1990. – (12): 65–68.
3. Gololobov V. G. Bone tissue regeneration at healing of bullet fractures. “Petersburg – XXI century”. – 1997. – 160.
4. Eremeev A. V., Svetlakov A. V., Bolshakov I. N., Vlasov A. A., Arapova V. A. Functions of cultivated embryonic cells on collagen-chitozan
matrix. Cellular transplantation and tissue engineering. – 2009. – 2: 55–62.
5. Ozerskaya O. S., Shegolev V. V. Experimental approach to substantiation of applying cellular compositions on the base of fibroblasts in
dermatocosmetology. Cellular transplantation and tissue engineering. – 2008. – 2: 66–67.
6. Shved Yu. L., Kukhareva L. V. et all. Design of polymeric substrate for human skin fibroblasts cultivation. Cytology. – 2006. – 2:161–168.
7. Kassin V. Yu., Aboyants R. K., Mironova L. L., Konyushko O. I. et all. Experimental study of connective tissue reaction on hydrogel implantation
with incorporated fibroblasts. Biotechnology. – 2004. – 6: 71–75.
8. Rasulov M. F. Use of marrow mesenchymal stem cells and embryonic fibroblasts in treatment of burn wounds. Pacific Med J. – 2004. –
9. Sonobe M., Hattori K., Tomita N., Yoshikawa Т., Aoki H., Takakura Y., Suguro T. Stimulatory effects of statins on bone marrow-derived
mesenchymal stem cells. Study of a new therapeutic agent for fracture. Biomed Mater Eng. – 2005. – 15 (4):261.
10. Lin C., Sun J. S., Hou S. M. External fixation with or without supplementary intramedullary Kirschner wires in the treatment of distal
radial fractures. Can J Surg. – 2004. – 47 (6):431–7. – PMID: 15646442.
11. Mangano C., Bartolucci E. G., Mazzocco C. A new porous hydroxyapatite for promotion of bone regeneration in maxillary sinus augmentation:
clinical and histologic study in humans. Int. J. OralMaxillofac. Implants. – 2003. – 18:23–30.
12. Qu S. X., Guo X., Weng J., Cheng J. C. Y., Feng В., Yeung H. Y. Zhang X. D. Evaluation of the expression of collagen type I in porous
calcium phosphate ceramics implanted in an extra-osseous site. Biomaterials. – 2004. – 25: 659–667.
13. Urazmetova M. D. Treatment methods of burns and injuries. Patent № 4995. Human embryonic fibroblasts received by prof. Urazmetova
M. D. – 1997.