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European Science Review, Issue 9-10/2016

Bacteriocin production by Lactobacillus plantarum 42 strain

DOI: https://doi.org/10.29013/ESR-16-9.10-17-20

Pages: 17 - 20

Authors: Miralimova S. M., Ogay D. K., Ibragimova A. A., Kutlieva G. D.

Abstract: Bacteriocins are ribosomally synthesized antibacterial peptides secreted by certain types of bacteria and active against both closely related species, and members of other species. Currently bacteriocins are recommended for use as antimicrobial agents in the food industry and in medicine. Bacteriocin production significantly depends on several factors such as culture conditions — pH, temperature, composition of the growth medium and the growth phase of producer strain. Bacteriocins can both be released to the culture medium, and remain attached to the producer cell. Optimization of growth conditions for bacteriocin production and increase its activity is of great economic importance to reduce its production cost. The aim of this study was to determine the localization of a bacteriocin of Lactobacillus plantarum 42, active against Enterococcus faecalis and to determine the optimal culture conditions in which there is its maximum output has been observed. The Lactobacillus plantarum 42 strain synthesizes bacteriocin, which is active against Enterococcus faecalis, is released into a solid and a liquid nutrient medium, but found only at 10 times the concentration in MRS broth. Bacteriocin detected at early stationary growth phase (18 hours) and remains active until 76 hours after initiation of fermentation. Maximal amount of bacteriocin was detected after 48 hours of fermentation at the initial pH value of growth media 6. There was no difference in the cultivation temperatures of 30oC and 37oC for bacteriocin production. This bacteriocin proved to be a secondary metabolite.

Keywords: Lactobacillus plantarum 42; bacteriocin; Enterococcus faecalis

Bibliography:
1. Leroy F. and De Vuyst L. Lactic Acid Bacteria as Functional Starter Cultures for the Food Fermentation Industry. Trends in Food Science
& Technology, – Vol. 15, – No. 2, – 2004. P. 67–78.
2. Riley M. A. and Wertz J. E. Bacteriocins: Evolution, Ecology, and Application. Annual Review of Microbiol-ogy, – Vol. 56, – No. 3, –
2002. – P. 117–137.
3. Nes I. F., Yoon S. S., Diep D. B. Ribosomally synthesized antimicrobial peptides (bacteriocins) in Lactic acid bacteria. Food sci biotechnol.
– Vol.16, – No 5, – 2007. P. 675–690.
4. Papagianni M. Ribosomally Synthesized Peptides and Antimicrobial Properties: Biosynthesis, Structure, Func-tion, and Applications.
Biotechnology Advances, – Vol. 21, – No. 6, – 2003. – P. 465–499.
5. Tagg J. R., Dajani A. S. and Wannamaker L. W. Bacteriocins of Gram-Positive Bacteria. Bacteriological Reviews, – Vol. 40, – No. 3, 1976.
P. 722–756.
6. Ennahar S., Sonomoto K. and Ishizaki A. Class IIa Bacteriocins from Lactic Acid Bacteria: Antibacterial Activity and Food Preservation.
Journal of Bioscience and Bioengineering, – Vol. 87, – No. 6, – 1999. – Pp. 705–716.
7. Paul Priyesh Vijayakumar and Peter M. Muriana. A Microplate Growth Inhibition Assay for Screening Bacteriocins against Listeria
monocytogenes to Differentiate Their Mode-of-Action. Biomolecules. – Vol. 5, – 2015. – P. 1178–1194.
8. Des Field, Paula M. O. Connor, Paul D. Cotter, Colin Hill1, R. Paul Ross. The generation of nisin variants with enhanced activity against
specific Gram-positive pathogens. Molecular Microbiology – Vol. 69 (1), – 2008. – P. 218–230.
9. Miralimova Sh. M., Ogay D. K., Elova N. A., Sokhibnazarova Kh. A., Kutliyeva G. D., Shakirova D. N. Probiotic properties of the Lactobacillus
plantarum bacteriocinogenic strain. Pharmaceutical Journal. – No 2, – 2016. – P. 111–116.
10. Miralimova Sh. M., Ogay D. K., Sokhibnazarova Kh. A., Kutliyeva G. D. Isolation and selection of lactic acid bacteria antagonistic to
enterococci. Black Sea scientific journal of academic research. – Vol 25, – Issue 07, – 2015. – P. 31–34.
11. Harris L. J., Daescheyl M. A., Stiles M. E., Klaenhammer T. R. Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes.
J Food Prot, – Vol. 52, – 1999. Р. 384–387.
12. Balouiri M., Sadiki M., Ibnsouda S. K. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical
Analysis. – Vol. 6, – No 2, – 2016. – P. 71–79.
13. MUK 4.2.2602–10. 4.2. Control methods. Biological and microbiological factors. System of preregistration preclinical evaluation of
safety of the preparations. Selection, evaluation and maintaining of industrial strains used in probiotics production. Guidelines. – 2011.
14. Dufour A., Hindre T., Haras D., Le Pennec J. P. The biology of lantibiotics from the lacticin 481 group is coming of age. FEMS Microbiol.
Rev. – Vol. 31, – 2007. – P. 134–167.
15. Todorov S. D., Dicks L. M. T. Effect of growth medium on bacteriocin production by Lactobacillus plantarum ST194BZ, a strain isolated
from Boza. Food Technol. Biotechnol. – Vol. 43 (2), – 2005. – P. 165–173.
16. Muller D. M., Carrasco M. S., Tonarelli G. G. and Simonetta A. C. Characterization and purification of a new bacteriocin with a broad inhibitory
spectrum produced by Lactobacillus plantarum lp 31 strain isolated from dry-fermented sausage. Journal of Applied Microbiology,
– Vol. 106, – 2009. – P. 2031–2040.
17. Paynter M. J. B., Brown K. A., Hayasaka S. S. Factors affecting the production of an antimicrobial agent, plantaricin F, by Lactobacillus
plantarum BF001. Letters in applied microbiology, – Vol. 24, – 1997. – P. 159–165.
18. Barefoot S. F., Klaenhammer T. R. Purification and characterization of the lactobacillus acidophilus bacteriocin, lactacin B. Antimicrobial
agent and chemotherapy, – Vol. 26, – 1984, – Pp. 328–334.
19. West C. A., Warner P. J. Plantacin B, a bacteriocin produced by Lactobacillus plantarum NCDO 1193. FEMS microbiology letters. Vol.
49, 1988. Pp. 163–165.
20. Tagg J. R., Dajani A. S. and Wannamaker L. W. Bacteriocins of Gram-Positive Bacteria. Bacteriological Reviews, – Vol. 40, – No. 3,
1976. – P. 722–756.
21. Jimenez-Diaz R., Rios-Sanchez R. M., Desmazeaud M., Riuz-Barba J. L. Plantaricins S and T, two new bacteriocins produced by Lactobacillus
plantarum LPC010 isolated from a green olive fermentation. Applied and environmental microbiology. – Vol. 59, – 1993. P.
1416–1424.
22. Daeschel M. A., McKenney M. C., McDonald L. C. Bacteriocidal activity of Lactobacillus plantarum C‑11. FOOD MICROBIOLOGY, –
Vol. 7, – 1990. –P. 91–98.
23. Lewus C. B., Montville T. J. Further characterization of bacteriocins plantaricin BN, Bavaricin MN and pediocin A. Food biotechnology,
– Vol. 6, – 1992. – P. 153–174.
24. Gonzales B., Arca P., Mayo B., Suarez J. E. Detection, purification and partial characterization of plantaricin C, a bacteriocin produced
by a Lactobacillus plantarum strain of dairy origin. Applied and environmental microbiology. – Vol. 60, – 1994. – P. 2158–2163.
25. Yang R., Johnson M. C., Ray B. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Applied and environmental
microbiology. – Vol. 58, – 1992. – P. 3355–3359.
26. Prema P. In vitro antagonistic activity of probiotic Lactobacillus plantarum against water borne pathogens. International Journal of
pharmacy and pharmaceutical sciences. – Vol. 5 Issue 4. – 2013. – P. 175–178.