Pages: 17 - 20
Authors: Miralimova S. M., Ogay D. K., Ibragimova A. A., Kutlieva G. D.
Abstract: Bacteriocins are ribosomally synthesized antibacterial peptides secreted by certain types of bacteria and active against both closely related species, and members of other species. Currently bacteriocins are recommended for use as antimicrobial agents in the food industry and in medicine. Bacteriocin production significantly depends on several factors such as culture conditions — pH, temperature, composition of the growth medium and the growth phase of producer strain. Bacteriocins can both be released to the culture medium, and remain attached to the producer cell. Optimization of growth conditions for bacteriocin production and increase its activity is of great economic importance to reduce its production cost. The aim of this study was to determine the localization of a bacteriocin of Lactobacillus plantarum 42, active against Enterococcus faecalis and to determine the optimal culture conditions in which there is its maximum output has been observed. The Lactobacillus plantarum 42 strain synthesizes bacteriocin, which is active against Enterococcus faecalis, is released into a solid and a liquid nutrient medium, but found only at 10 times the concentration in MRS broth. Bacteriocin detected at early stationary growth phase (18 hours) and remains active until 76 hours after initiation of fermentation. Maximal amount of bacteriocin was detected after 48 hours of fermentation at the initial pH value of growth media 6. There was no difference in the cultivation temperatures of 30oC and 37oC for bacteriocin production. This bacteriocin proved to be a secondary metabolite.
Keywords: Lactobacillus plantarum 42; bacteriocin; Enterococcus faecalis
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